Journal: Diabetes

Article Title: Amyloid-β Induces Hepatic Insulin Resistance In Vivo via JAK2

doi: 10.2337/db12-0670

Figure Lengend Snippet: Neutralization of Aβ downregulates fasting blood glucose and improves insulin sensitivity in APP/PS1 AD model mice. A : Fasting blood glucose levels of APP/PS1 mice intraperitoneally injected once weekly with control IgG, 1H3, or 6C8 anti–Aβ-neutralizing antibody for 4 months ( n = 4 for each group). B and C : Fasting blood glucose levels were measured after fasting for 4 h. Fasting blood glucose ( B ) and plasma insulin ( C ) of APP/PS1 mice intraperitoneally injected once weekly with control IgG ( n = 3) or 6C8 anti-Aβ antibody ( n = 4) for 9 months. Blood glucose and insulin levels were measured after fasting for 4 h. D : HOMA-IR was calculated from fasting glucose and insulin levels in panels B and C . Data are presented as mean and SD. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Control IgG, 1H3, and 6C8 mouse anti-human Aβ monoclonal antibodies were from Anogen-Yes Biotech Laboratories (Canada).

Techniques: Neutralization, Injection

Journal: Diabetes

Article Title: Amyloid-β Induces Hepatic Insulin Resistance In Vivo via JAK2

doi: 10.2337/db12-0670

Figure Lengend Snippet: Neutralization of Aβ inhibits hepatic JAK2/STAT3/SOCS1 signaling in APP/PS1 AD model mice. A : Immunoblot analysis of liver JAK2 and STAT3 phosphorylation states and SOCS-1 protein levels in APP/PS1 mice injected with control IgG or anti-Aβ 1H3 antibody for 4 months. B : Quantification of phosphorylated JAK2 and STAT3 levels and SOCS-1 protein levels in panel A . C : Immunoblot analysis of liver JAK2 and STAT3 phosphorylation states and SOCS-1 protein levels in APP/PS1 mice injected with control IgG or anti-Aβ 6C8 antibody for 9 months. D : Quantification of phosphorylated JAK2 and STAT3 levels and SOCS-1 protein levels in panel C . All protein levels were normalized to tubulin. Data are presented as mean and SD. * P < 0.05.

Article Snippet: Control IgG, 1H3, and 6C8 mouse anti-human Aβ monoclonal antibodies were from Anogen-Yes Biotech Laboratories (Canada).

Techniques: Neutralization, Western Blot, Injection